Coding

Part:BBa_K1991005

Designed by: Chen, Pei-En   Group: iGEM16_Mingdao   (2016-10-12)

LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)

AOX1 gene (BBa_K1991000) was fused with Lpp-OmpA (BBa_K1991004) on pSB1C3. Lpp-OmpA can direct AOX1 protein on E. coli cell surface.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2437
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 436
    Illegal XhoI site found at 2446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2128


[edit]
Categories
Parameters
None